Export of GlossWell Type Anti-Viral from Japan

Please let more people know about this page! 
GlossWell #360 / #240 / #750 Type Anti-Viral is a paint. Since paint is classified as a hazardous material internationally, the cost of exporting it from Japan by air or sea is very high, and it is very difficult for an individual to handle the payment of freight and customs clearance for importation.

In this page, we are announcing a special paint that contains a very effective anti-bacterial compound against COVID-19 : GlossWell #360 / #240 / #750 Type Anti-Viral to corporations, trading companies and government officials in your country. We are disclosing all the necessary evidence to prove the efficacy against COVID-19. All of this evidence has been tested and obtained by public institutions in Japan.

GlossWell #360 / #240 / #750 Type Anti-Viral forms a very tough and special coating with anti-bacterial properties. The coating will adhere to the surface for a long time. If a similar special coating does not exist in your country, please inform any person or entity that can import GlossWell #360 / #240 / #750 Type Anti-Viral or any government official of the existence of this page!

List of various test data

Various tests on the anti-virus and anti-bacterial performance of this coating were conducted at the Kobe Testing Center / Microorganism Testing Laboratory of the Japan Textile Products Quality Technology Center. Details of the test results are shown below.

General Incorporated Foundation : Japan Textile Products Quality Technology Center

Microorganisms to be tested

  • Virulence test: influenza A virus (with envelope)
  • Antiviral test : Feline calicivirus (without envelope membrane)
  • Antibacterial test : Pseudomonas aeruginosa
  • Antibacterial test : E. coli (O157:H7)
  • Antifungal test : Black mold

Antiviral test: Influenza A virus (with an envelope membrane)

◯Specimen: April 2, 2020 / Reply date: June 5, 2020
◯ Test item: Antiviral test
◯ Test details: Evaluate the antiviral properties of polycarbonate sheets
◯ Test method: ISO21702 / Measurement of antiviral activity on plastics and other non-porous surfaces
◯ Testing organization: Microbiology Laboratory, Kobe Testing Center, Japan Textile Quality Technology Center

[ Test Summary ]
・Study virus: Influenza A virus (H3N2) A/Hong Kong/8/68; TC adapted ATCC VR-1679
・Host cells : MDCK cells (canine kidney-derived cells)
・Test sample
(1) GlossWell #360 Type Anti-Viral / Polycarbonate sheet (unprocessed) / control: Sample submitted by the client
(2) GlossWell #360 Type Anti-Viral / Polycarbonate Board (Processed)
・SCDLP medium
・Leaving conditions : Leaving temperature 25°C
・Time of storage 24 hours :  (1) GlossWell #360 Type Anti-Viral polycarbonate sheet (unprocessed) was measured immediately after leaving the product for 24 hours.
・Sample size : 5cm x 5cm
・Adhesion film: Polyethylene (4cm x 4cm)
・Inoculation volume of the test virus suspension: 0.4 mL
・Cleanliness of the test specimens: Not conducted.

[ Test Operation: Main Test ]
1. Infect host cells with the virus and culture them, and remove the remaining cells by centrifugation to make a virus suspension.
2. dilute the virus suspension 10-fold with sterile distilled water to a concentration of 1-5X10∧7 PFU/mL, and treat as a test virus suspension.
3. Place each specimen on the bottom of a sterile petri dish with the processed side up, and inoculate it with 0.4 mL of the test virus suspension. 
4. Cover the Petri dish with adhesive film and press down lightly so that the test virus suspension is spread over the entire film.
5. Cover the petri dish with the lid.
6. Add 10 mL of washout solution to each test specimen after 24 hours at 25°C. 
7. Scrape the surface of each test specimen and the contact film to remove the virus.
8. Measure the viral titer by the plaque assay.

[ Host cell verification test ]
2 )-1 Cytotoxicity confirmation test
1 ) Add 10mL of washout solution to each test specimen, and perform the washout procedure as in this test. 2) Stain cells as in the plaque assay, and confirm the presence of cytotoxicity.
2 ) Stain cells in the same way as the plaque assay and confirm the presence or absence of cytotoxicity.
2 )-2 Confirmation of cell susceptibility to viruses
1. Add 10mL of washing-up liquid to each test specimen, and perform washing-up operation as in this test. 
2. 5 mL of the above washout solution is placed in a sterile test tube.
3. Prepare 4-6 X 10 ∧ 4 PFU/mL test virus suspension, and add 0.05 mL of the suspension to the washout solution (2).
4. let it stand at 25°C for 30 minutes.
5. confirm the susceptibility of the cells to the virus by measuring the viral infection titer by the plaque assay.

[ Test method ]
1 ) Main test
・Test virus: Influenza A virus (H3N2), A/Hong Kong/8/68; TC adapted ATCC VR-1679
・The concentration of the test virus suspension: 3.5×10∧7 PFU/ml

( Note 4 ) SCDLP medium was used as a negative control.

[ Conditions for acceptance of the study ]
2-1) Cytotoxicity : None
2-2) Confirmation of cell susceptibility to virus : | Sn – Su | ≤ 0.5 and | Sn – St | ≤ 0.5

Testing organization: Microbiological Testing Laboratory, Kobe Testing Center, Japan Textile Quality Technology Center

Antiviral test: feline calicivirus (without envelope)

[ Exam summary ]
・ Test virus: Feline calicvirus; Strain: F-9 ATCC VR-782
・ Host cells: CRFK cells (cat kidney-derived cells)
・ Test sample: ① Paint GlossWell # 360 Type Anti-Viral / ② Glass plate
・ Washing solution: SCDLP medium supplemented with Fetal Bovine Serum to a final concentration of 10%
・ Adhesive film: polyethylene (4cm × 4cm)

Norovirus is rare for cell culture, so it is not possible to test, measure and evaluate the effect of various disinfectants or individual concentrations. For that reason, caliciviruses and mouse norovirus, which are closely related, are our testing, measuring and evaluating the disinfecting effect of general disinfectants.

[ Test method ]
1 ) Main test
1. Prepare the test virus suspension.
2. Place the sterilizing agent filter paper on the bottom of the sterile petri dish, add 4.5 mL of sterile ion-exchanged water, place a U-shaped glass tube so that the test piece does not touch the filter paper for humidity control, and place a processing surface on it. Place the test sample on top.
3. Inoculate 0.4 mL of the test virus suspension into each sample.
4. Cover with the adhesive film and press gently so that the test virus suspension spreads over the entire film.
5. Cover the Petri dish.
6. After leaving at ℃ 25 ℃ for 24 hours put the specimen into the sterilizer stomacher bag, and wash out the virus from the specimen by adding 10mL of washing solution.
7. Measure the virus infectivity by the plaque assay.

2 ) Host cell verification test:
2 ) -1 Cytotoxicity confirmation test
1. Put the sample in a sterilizer stomacher bag, add 10 mL of the washing solution, and perform the washing operation in the same manner as in this test.
2. Incubate at room temperature for 30 minutes.
2. Stain the cells in the same manner as in the plaque assay and check for cytotoxicity.

2 ) -2 Confirmation test of cell susceptibility to -2 virus
1. Put the sample in a sterilizer stomacher bag, add 10 mL of the washing solution, and perform the washing operation in the same manner as in this test.
2. Transfer 5 mL of the above washing solution to a sterilized test tube.
3. Prepare the test virus suspension at 5 x 104 PFU / mL, and add 0.05 mL of the suspension to the washing solution in step 2.
4. Leave at room temperature for 30 minutes.
5. Measure the virus infectivity by the -plaque assay to confirm the sensitivity of the cells to the virus.

[ Test result ]
1 ) Main test
Test virus suspension: Feline calicvirus 1.0 x 107 PFU / mL

2 ) Host cell verification test:

( Note 1 ) ① A glass plate was used as a control sample.
( Note 2 ) PFU: plaque forming units

2 ) -1
From the results of the cytotoxicity confirmation test, no cytotoxicity was confirmed in any of the samples. In addition, from the results of the test for confirming the sensitivity of the cells to 2) -2 virus, no remarkable decrease in the sensitivity of the cells to the virus was observed in any of the samples.

Testing organization: Microbiological Testing Laboratory, Kobe Testing Center, Japan Textile Quality Technology Center

Antibacterial test / Pseudomonas aeruginosa

[ Test method ]
* Antibacterial test JIS Z 2801 (Film adhesion method)
・ Test strain: Pseudomonas aeruginosa NBRC3080
・ Bacterial solution adjustment solution: 1 / 500NB medium
・ Test bacterial solution inoculation volume: 0.4ml
・ Unprocessed sample: polyethylene film

[ Test results ]

( Note 1 ) A polyethylene film was used as a non-processed test piece.
( Note 2 ) Antibacterial activity value R = Ut-At

Testing organization: Microbiological Testing Laboratory, Kobe Testing Center, Japan Textile Quality Technology Center

Antibacterial test / Escherichia coli (O157: H7)

[ Test method ]
* Antibacterial test JIS Z 2801 (Film adhesion method)
-Test strain: Escherichia coli (serotype II O157: H7, verotoxin type I and type II producing strains)
・ Escherichia coil RIMD 0509952
・ Bacterial solution adjustment solution: 1 / 500NB medium
・ Test bacterial solution inoculation volume: 0.4ml
・ Unprocessed sample: polyethylene film

[ Test results ]

( Note 1 ) ① A polyethylene film was used as a non-processed test piece.
( Note 2 ) Antibacterial activity value R = Ut-At

Testing organization: Microbiological Testing Laboratory, Kobe Testing Center, Japan Textile Quality Technology Center

Antifungal test / Black mold

[ Test method ]
* Antibacterial test JIS Z 2801 (Film adhesion method)
・ Test strain: Cladosporium cladosporioides NBRC6348 (Black mold)
・ Measurement method: Luminescence measurement method
・ Spore suspension preparation solution: 1/20 SDB medium
・ Spore suspension inoculation volume: 0.4ml
・ Mold spore concentration: 1.0 × 105spores / ml
・ Culture conditions: 25 ° C, 95% RH, 42 hours
・ Unprocessed sample: polyethylene film

[ Test results ]

( Note 1 ) Antifungal activity value [FS] = (Fb-Fa) – (Fc-Fo)
( * 2 ) Growth value [F] = Fb-Fa

Testing organization: Microbiological Testing Laboratory, Kobe Testing Center, Japan Textile Quality Technology Center

Film performance

Material: Bond steel plate / Film thickness: 6-8μm / Curing condition: After drying at 80 ℃ for 30 minutes, leave at room temperature for 5 days
* The above values are reference values and not standard values.

Precautions for painting

○ Coating environment: Avoid using in an environment with poor ventilation.
○ Pretreatment: Oil, water, and dirt on the surface of the material should be sufficiently removed by solvent degreasing.
○ Painting: Paint immediately. Leaving it for a long time may cause clogging and uneven coating.
・ Manage the film thickness within the specified range.
○ Drying: Organic gas is generated during drying, so provide sufficient ventilation / exhaust.
○ Storage: Store the paint in a cool and dark place.
・ This paint has the property of reacting with moisture in the air. Please seal it after use.
○ Disposal: Follow the MSDS (Material Safety Data Sheet) for disposal of paint residue and waste liquid.
○ Handling Precautions: Do not use in a place with a fire because it uses a flammable organic solvent.
・ Be careful not to come in contact with skin and mucous membranes, especially eyes, as they are irritating.
・ In case of contact, wash with plenty of water.
○ Others: Refer to the product MSDS for details.

Antiviral and antibacterial test results & Documents

Technical and test antiviral and antibacterial test reports.pdf

Inactivation test data

Announcement on Quaternary Ammonium Salts by METI of Japan

SDS Down Load

SDS / ENGLISH : GlossWell #360 Type Anti-Viral Down Load

Recruiting Distributors & Dealers

Our company offers numerous special paints made using cutting edge Japanese chemical technology. Each one of our paints excels at repelling water and oil and withstands submersion. Furthermore, they are also designed to be ultra bright, hard, UV resistant, and heat resistant as well as aesthetically superior. We can offer high added value along with unprecedented new ideas for final surface finishing on all kinds of base materials. We are currently recruiting overseas distributors to carry these superior Japan-made special paints. If you are interested, please contact us through our Contact Form.

Recruiting Distributors & Dealers